| 存贮条件 | 储存温度2-8℃ |
| 应用 | Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA.Removal endotoxins bound to cationic proteins such as lysozyme and ribonuclease A.Reported useful for the isolation of hepatic, yeast, and mung bean mitochondriaDetermination of enzyme localization on membranesTreatment of paraffin embedded tissue sections to expose antigen binding sites for antibody labeling.Digestion of proteins from brain tissue samples for prions in Transmissible Spongiform Encephalopathies (TSE) research. |
| 产品介绍 | Proteinase K (PROK) is a serine protease with broad specificity towards aliphatic, aromatic and other hydrophobic amino acids. PROK has a molecular weight of 27,000 daltons and is Ca2+dependent. It is not inactivated by metal ion chelating agents such as EDTA, sulfhydryl reagents, PCMB, TLCK, or TPCK. It also retains activity in 0.5% SDS. It can be inhibited by PMSF or DFP. |
| 备注 | A lyophilized powder. Purified to remove DNase and RNase. |
| 生化机理 | Proteinase K is a stable and highly reactive serine protease. Evidence from crystal and molecular structure studies indicates the enzyme belongs to the subtilisin family with an active-site catalytic triad (Asp39-His69-Ser224). It is stable in a broad range of environments: pH, buffer salts, detergents (SDS), and temperature. In the presence of 0.1-0.5% SDS, proteinase K retains activity and will digest a variety of proteins and nucleases in DNA preparations without compromising the integrity of the isolated DNA. |
| 别名 | Proteinase KEndopeptidase K |
蛋白酶K 来源于林伯氏白色念球菌
Proteinase K from Tritirachium album limber （订货以英文为准）
编号：P128666
CAS号：39450-01-6
分子式：C29H27N2O12P
分子量：626.50
| 产品名称 | Proteinase K from Tritirachium album limber |
| 中文名称 | 蛋白酶K 来源于林伯氏白色念球菌 |
| CAS号 | 39450-01-6 |
| 分子式（M.F.） | C29H27N2O12P |
| 分子量（M.W.） | 626.50 |
| 储存条件 | 2-8°C储存 |
| RTECS | SZ5999500 |
| WGK德国 | 1 |
搜索质检报告(COA)
搜索MSDS
相关产品
¥ 103.90
T117926-25ml
¥ 1441.90
H128453-1g
¥ 2266.90
H128454-10UN
¥ 557.90
A118327-100mg
¥ 680.90
M139683-5KU
党沛 
