概述
Exendin-4 is a novel 39-amino acid peptide isolated from the venom of the Gila monster Heloderma suspectum. It shares 53 % sequence homology with GLP-17-36amide and interacts with the same membrane receptor. Exendin-4 enhances glucose-dependent insulin secretion, suppresses inappropriately elevated glucagon secretion, and slows gastric emptying in vivo. It also promotes -cell proliferation and neogenesis in vitro and in animal models. Recombinant Exendin-4 is E. coli expression of a synthetic DNA sequence encoding the 39 amino acid of Exendin-4.
使用说明
This material is offered by Sangon Biotech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
技术规格
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Purity | > 96 % by SDS-PAGE and HPLC analyses. |
| Source | Escherichia coli. |
| Biological Activity | 1. Regulates Glucose levels rapidly; 2. Reduces Insulin resistance; 3. Reduces Glucagon; 4. Reduces HbA1c; 5. Stimulates beta cell growth which stimulates insulin production. |
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4. |
| Endotoxin | Less than 10 EU/mg of rExendin-4 as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| AA Sequence | HGEGTFTSDL SKQMEEEAVR LFIEWLKNGG PSSGAPPPS |
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