概述
Streptavidin is a tetrameric protein composed of identic subunits. Each subunit binds one biotin molecule with a KD of ~1×10-15 M. The preparation contains an N- and C-terminal shortened variant (core streptavidin) with improved properties concerning homogeneity, solubility, resistance towards proteolytic degradation and accessibility of the biotin binding pocket as compared to native streptavidin.
使用说明
This material is offered by Sangon Biotech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
技术规格
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Purity | > 98 % by SDS-PAGE and HPLC analyses |
| Source | Recombinant streptavidin from Streptomyces avidinii, produced in Escherichia coli. |
| Specific Activity | > 17 U/mg (one unit binds 1 μg D-biotin at pH 8.9) |
| Formulation | Lyophilized in 10 mM potassium phosphate buffer pH 6.5. |
| Endotoxin | Less than 0.1 EU/μg of rStreptavidin as determined by LAL method. |
| Reconstitution | Dissolve with double distilled water |
| AA Sequence | MAEAGITGTW YNQLGSTFIV TAGADGALTG TYESAVGNAE SRYVLTGRYD SAPATDGSGT ALGWTVAWKN NYRNAHSATT WSGQYVGGAE ARINTQWLLT SGTTEANAWK STLVGHDTFT KVKPSAAS |
| Shipment | At ambient temperature |
| A282 of 0.1% solution | 3.1 |
| Proteolytic Activity | < 10-3 U/mg protein (Azocoll, 25 °C, 24 h, pH 8.0) |
| Solubility | Clearly soluble in ddH2O (OD405nm < 0.1 at 10 mg/ml water) |
| Stability (lyophilized) | 2 years after shipping |
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