概述
Recombinant Human Copine-1 is produced by our E.coli expression system and the target gene encoding Met1-Ala537 is expressed with a 6His tag at the N-terminus, 6His tag at the C-terminus.
使用说明
This material is offered by Sangon Biotech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
技术规格
| Tag | N, C-6His |
| 种属 | Human |
| 表达系统 | E.coli |
| Accession# | Q99829 |
| Source | E.coli |
| Formulation_Description | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
| Storage | Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
| Reconstitution | Dissolve the lyophilized protein in distilled water. |
| Purity | Greater than 95% as determined by reducing SDS-PAGE. |
| Endotoxin | Less than 0.1 ng/μg (1 EU/μg) as determined by LAL test. |
| Background | Copine-1(CPNE1) encodes a calcium-dependent protein which belongs to the copine family. CPNE1contains two N-terminal type II C2 domains and an integrin A domain-like sequence in the C-terminus. However, CPNE1 does not contain a predicted signal sequence or transmembrane domains. CPNE1 may regulate molecular events at the interface of the cell membrane and cytoplasm. CPNE1 has a broad tissue distribution and it may function in membrane trafficking. |
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