概述
Fusion protein corresponding to a region derived from 319-541 (V367F) amino acids of human SARS-CoV-2 S protein
技术规格
| Full name: | Severe acute respiratory syndrome coronavirus 2 spike glycoprotein Receptor-binding domain |
| Synonyms: | COVID-19; 2019-nCoV; SARS-CoV-2; spike glycoprotein; Spike RBD Protein; 新型冠状病毒 |
| Swissprot: | P0DTC2 |
| Gene Accession: | MN908947 |
| Purity: | >90%, as determined by Coomassie blue stained SDS-PAGE |
| Expression system: | Mammalian cell |
| Tags: | 10xHis tag C-terminals |
| Species: | SARS-CoV-2/2019-nCoV |
| Molecular mass: | 30.1 kDa |
| Research areas: | Microbiology |
| Activity: | Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD (V367F) at 5 μg/ml can bind human ACE2 (Catalog number: D140021), the EC50 is 65.58-90.16 ng/ml. |
| Endotoxin: | Less than 1.0 EU/ug as determined by LAL method. |
| Reconstitution: | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
| Background: | The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. |
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