应用
The Protein A molecule contains four high-affinity (Ka =108/mole) binding sites capable of interacting with the Fc region from IgG of several species including human and rabbit. Optimal binding occurs a pH 8.2, although binding is also good at neutral or physiological conditions (pH 7.0-7.6).
使用说明
This material is offered by Sangon Biotech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
技术规格
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Purity | > 97 % by SDS-PAGE analyses. |
| Source | Escherichia coli. |
| Formulation | Lyophilized with no additive. |
| Endotoxin | Less than 0.1 EU/μg of Protein A, His as determined by LAL method. |
| Reconstitution | Dissolve in distilled water or saline. |
| Specificity | The interaction between Protein A and IgG is not equivalent for all species. Even within a species, Protein A interacts with some subclasses of IgG and not others. For instance, human IgG1, IgG2 and IgG4 bind strongly, while IgG3 does not bind. There are |
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