应用
Protein A/G is an excellent tool for purification and detection of mouse monclonal antibodies from IgG subclasses without interference from these other serum proteins. Individual subclasses of mouse monoclonals are most likely to have stronger affinity to this chimeric protein than to either Protein A or Protein G. Binding is less pH-dependent than either Protein A or Protein G alone, occurring well at pH 5-8.
使用说明
This material is offered by Sangon Biotech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
技术规格
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Purity | > 96 % by SDS-PAGE and RP-HPLC analyses. |
| Source | Escherichia coli. |
| Formulation | Lyophilized with no additive. |
| Endotoxin | Less than 0.1 EU/μg of Protein A/G, His as determined by LAL method. |
| Reconstitution | Dissolve in distilled water or saline. |
| Specificity | The extended Fc-binding properties of Protein A/G make it a popular tool in the investigation and purification of immunoglobulins. Protein A/G binds to all human IgG subclasses, IgA, IgE, IgM and to a lesser extent IgD; however, it does not bind mouse IgA |
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