概述
Recombinant Human cTn I is produced by E.coli expression system and the target gene encoding Met1-Ser210 is expressed with a 6His tag at the N-terminus.
使用说明
This material is offered by Sangon Biotech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
技术规格
| Taq | C-6His |
| 种属 | Human |
| 表达系统 | E.coli |
| Accession# | P19429 |
| Source | E.coli |
| Formulation_Description | Supplied as a 0.2 μM filtered solution of 20mM Tris-HCl, 100mM NaCl, 1mM DTT, 2M Urea, 10% Glycerol, pH 8.0. |
| Storage | Reconstituted protein solution should be stored at ≤ -20°C. |
| Purity | Greater than 95% as determined by reducing SDS-PAGE. |
| Endotoxin | Less than 0.1 ng/μg (1 EU/μg) as determined by LAL test. |
| Background | Troponin is a heterotrimer that regulates muscle contraction in skeletal and cardiac muscle (but not in smooth muscle). Troponin acts with intracellular calcium to control the interaction of actin and myosin filaments in striated muscle fibers. Though they perform similar functions, cardiac and skeletal troponins differ in sequence and can be differentiated in immunoassays. When muscle tissue is damaged, the troponin-tropomyosin complex breaks down and troponin I and troponin T are released into the blood. Cardiac troponin T (cTnT) and cardiac troponin I (cTnI) can be readily distinguished from their skeletal muscle analogs allowing confirmation of cardiac muscle tissue damage over skeletal muscle tissue damage. Troponins are excellent biomarkers for myocardial injury in cardiotoxicity because of the demonstrated tissue-specificity of cardiac and skeletal troponins. |
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