概述
Iodoacetyl-LC-Biotin is long-chain, sulfhydryl-reactive biotinylation reagents.For the reagent,reaction of the iodoacety1 group with a sulfhydryl (-SH) group is rapid and specific, especially when only a slight reagent-to-sulfhydrylmolar excess is used and the reaction is performed at pH 8.3 (7.5-8.5).1-3 The reaction occurs by nucleophilic substitution of iodine with a thiol (sulfhydryl) group, resulting in a stable thioether bond (Figure 1). If thiols are unavailable on the molecule, reaction can occur with histidy1 side chains at pH 6.9-7.0, but the reaction must be allowed to proceed for at least one week. Iodoacetyl reagents also will react with amino groups at pH > 10.
应用
Iodoacetyl-LC-Biotin is not soluble in water and must be dissolved in an organic solvent such as DMF before further dilution in aqueous solutions.
组份
50 mg/bottle
技术规格
| Formula | C18H31IN4O3S |
| Net Mass Addition | 382.53 |
| Spacer arm | 27.1 |
| Purity | >95% on TLC |
| Solubility | > 2 mg/ml in DMF for subsequent dilution in water or buffer |
党沛 
