Species
|
Gene ID
|
Accession
|
Source
|
Length
|
MW
|
Tag
|
-
|
-
|
-
|
E. coli
|
-
|
-
|
-
|
About this Protein
|
Name
|
Recombinant Streptavidin (Recombinant Streptavidin; rStreptavidin);重组链霉亲和素
|
Synonyms
|
N/A
|
Purity
|
>98% by SDS-PAGE and HPLC analyses
|
Biological Activity
|
>17U/mg (one unit binds 1μg D-biotin at pH8.9)
|
Physical Appearance
|
Sterile Filtered White lyophilized (freeze-dried) powder.
|
Formulation
|
Lyophilized in 10mM potassium phosphate buffer pH6.5.
|
Endotoxin
|
Less than 0.1EU/μg of rStreptavidin as determined by LAL method.
|
Reconstitution
|
Dissolve with double distilled water.
|
Category
|
Others
|
Background
|
Streptavidin is a tetrameric protein composed of identic subunits. Each subunit binds one biotin molecule with a KD of ~1×10-15M. The preparation contains an N- and C-terminal shortened variant (core streptavidin) with improved properties concerning homogeneity, solubility, resistance towards proteolytic degradation and accessibility of the biotin binding pocket as compared to native streptavidin.
|
Amino Acid Sequence
|
MAEAGITGTW YNQLGSTFIV TAGADGALTG TYESAVGNAE SRYVLTGRYD SAPATDGSGT ALGWTVAWKN NYRNAHSATT WSGQYVGGAE ARINTQWLLT SGTTEANAWK STLVGHDTFT KVKPSAAS
|
包装清单:
产品编号
|
产品名称
|
包装
|
P5084-1mg
|
Recombinant Streptavidin
|
1mg
|
P5084-5mg
|
Recombinant Streptavidin
|
5mg
|
P5084-100mg
|
Recombinant Streptavidin
|
100mg
|
-
|
说明书
|
1份
|
保存条件:
-20℃或更低温度保存,至少一年有效。由于蛋白的每次冻融均会引起部分失活,所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液),须分装后-20℃或更低温度冻存,以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用,溶液中的蛋白很容易粘附在管壁上,并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein,如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点,使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。