Species
|
Gene ID
|
Accession
|
Source
|
Length
|
MW
|
Tag
|
Mouse
|
20308
|
P51670
|
E. coli
|
101aa
|
11.6kDa
|
-
|
About this Antibody
|
Name
|
Recombinant Murine MIP-1γ/CCL9/CCL10 (Recombinant Murine Macrophage Inflammatory Protein-1γ/CCL9/CCL10; rMuMIP-1γ/CCL9/CCL10);Recombinant Mouse MIP-1γ/CCL9/CCL10 ; 重组小鼠巨噬细胞炎症蛋白1γ/CCL9/CCL10
|
Synonyms
|
CCF18; MIP-1 gamma
|
Purity
|
>95% by SDS-PAGE and HPLC analyses.
|
Biological Activity
|
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration range of 0.1-10ng/ml.
|
Physical Appearance
|
Sterile Filtered White lyophilized (freeze-dried) powder.
|
Formulation
|
Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4.
|
Endotoxin
|
Less than 1EU/μg of rMuMIP-1γ/CCL9/CCL10 as determined by LAL method.
|
Reconstitution
|
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
|
Category
|
Chemokines
|
Background
|
Murine CCL9/10 is belonging to the CC chemokine family. It is encoded by the gene CCL9. MRP-2 (CCL9/10) was isolated from a murine macrophage cell line, RAW 264.7. MRP-2 shows a 51% sequence identity at the protein level to MRP-1 (C10) and 46% identity to MIP-1-alpha (CCL3). The functional receptor for CCL9/10 has been identified as CCR1. In contrast, other CCR1 ligands, CCL3/MIP-1alpha and CCL5/RANTES are considered to be primary CCR1 ligands in inflammatory responses. CCL9 can activate osteoclasts suggesting an important role for it in bone resorption. Murine CCL9/10 is 75% amino acid identical to rat CCL9/10.
|
Amino Acid Sequence
|
QITHATETKE VQSSLKAQQG LEIEMFHMGF QDSSDCCLSY NSRIQCSRFI GYFPTSGGCT RPGIIFISKR GFQVCANPSD RRVQRCIERL EQNSQPRTYK Q
|
包装清单:
产品编号
|
产品名称
|
包装
|
P6798-5μg
|
Recombinant Murine MIP-1γ/CCL9/CCL10
|
5μg
|
P6798-20μg
|
Recombinant Murine MIP-1γ/CCL9/CCL10
|
20μg
|
P6798-100μg
|
Recombinant Murine MIP-1γ/CCL9/CCL10
|
100μg
|
P6798-1mg
|
Recombinant Murine MIP-1γ/CCL9/CCL10
|
1mg
|
-
|
说明书
|
1份
|
保存条件:
-20℃或更低温度保存,至少一年有效。由于蛋白的每次冻融均会引起部分失活,所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液),须分装后-20℃或更低温度冻存,以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用,溶液中的蛋白很容易粘附在管壁上,并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein,如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点,使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。