溶解性 | solubility(H2O): soluble 1 mg/mL, clear, colorless |
存贮条件 | 储存温度-20°C |
应用 | 分离DNA和RNA期间使核苷酸酶蛋白失活。去除内毒素对阳离子蛋白的约束,如溶菌酶和核糖核酸酶。研究报告显示用于分离肝,酵母,绿豆线粒体测定细胞膜上的酶用于抗体标记,石蜡包埋组织切片中暴露抗原结合位点。消化脑组织样本中的蛋白质,用于朊病毒的传染性海绵状脑病(TSE)的研究 |
产品介绍 | 蛋白酶K是一个稳定,高活性丝氨酸蛋白酶。从晶体和分子结构研究的证据表明这种酶属于枯草杆菌家族,带有活性催化位点催化三键(Asp39,His69 - Ser224)。它是在宽的的环境范围内保持稳定:pH值,缓冲盐,去垢剂(SDS),和温度。在0.1-0.5% SDS存在下,在分离DNA中,蛋白酶K保持活性,消化各种蛋白质和核酸酶。 |
备注 | Unit Definition One unit will hydrolyze urea-denatured hemoglobin to produce color equivalent to 1.0 μmole of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent). |
生化机理 | Proteinase K is a stable and highly reactive serine protease. Evidence from crystal and molecular structure studies indicates the enzyme belongs to the subtilisin family with an active-site catalytic triad (Asp39-His69-Ser224). It is stable in a broad range of environments: pH, buffer salts, detergents (SDS), and temperature. In the presence of 0.1-0.5% SDS, proteinase K retains activity and will digest a variety of proteins and nucleases in DNA preparations without compromising the integrity of the isolated DNA. |
别名 | Proteinase K;蛋白酶 K ;肽链内切酶;;Endopeptidase KProk Protease KTritirachium album proteinase K |
蛋白酶K
Proteinase K (订货以英文为准)
编号:P109033
CAS号:39450-01-6
分子式:C29H27N2O12P
分子量:626.50
产品名称 | Proteinase K |
中文名称 | 蛋白酶K |
CAS号 | 39450-01-6 |
分子式(M.F.) | C29H27N2O12P |
分子量(M.W.) | 626.50 |
储存条件 | -20°C储存 |
个人防护设备 | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
WGK德国 | 3 |
搜索质检报告(COA)
搜索MSDS
相关产品