蛋白酶K

Proteinase K (订货以英文为准)

编号:P109033
CAS号:39450-01-6
分子式:C29H27N2O12P
分子量:626.50
货号 品牌 包装 目录价 您的价格 库存 数量 购买
P109033-25mg 阿拉丁 25mg ¥51.90
P109033-1g 阿拉丁 1g ¥598.90
P109033-100mg 阿拉丁 100mg ¥102.90
P109033-500mg 阿拉丁 500mg ¥330.90
P109033-25g 阿拉丁 25g ¥9785.90
P109033-100g 阿拉丁 100g ¥30797.90
P109033-5g 阿拉丁 5g ¥2390.90
产品名称 Proteinase K
中文名称 蛋白酶K
CAS号 39450-01-6
分子式(M.F.) C29H27N2O12P
分子量(M.W.) 626.50
储存条件 -20°C储存
溶解性solubility(H2O): soluble 1 mg/mL, clear, colorless
存贮条件储存温度-20°C
应用分离DNA和RNA期间使核苷酸酶蛋白失活。去除内毒素对阳离子蛋白的约束,如溶菌酶和核糖核酸酶。研究报告显示用于分离肝,酵母,绿豆线粒体测定细胞膜上的酶用于抗体标记,石蜡包埋组织切片中暴露抗原结合位点。消化脑组织样本中的蛋白质,用于朊病毒的传染性海绵状脑病(TSE)的研究
产品介绍蛋白酶K是一个稳定,高活性丝氨酸蛋白酶。从晶体和分子结构研究的证据表明这种酶属于枯草杆菌家族,带有活性催化位点催化三键(Asp39,His69 - Ser224)。它是在宽的的环境范围内保持稳定:pH值,缓冲盐,去垢剂(SDS),和温度。在0.1-0.5% SDS存在下,在分离DNA中,蛋白酶K保持活性,消化各种蛋白质和核酸酶。
备注Unit Definition One unit will hydrolyze urea-denatured hemoglobin to produce color equivalent to 1.0 μmole of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).
生化机理Proteinase K is a stable and highly reactive serine protease. Evidence from crystal and molecular structure studies indicates the enzyme belongs to the subtilisin family with an active-site catalytic triad (Asp39-His69-Ser224). It is stable in a broad range of environments: pH, buffer salts, detergents (SDS), and temperature. In the presence of 0.1-0.5% SDS, proteinase K retains activity and will digest a variety of proteins and nucleases in DNA preparations without compromising the integrity of the isolated DNA.
别名Proteinase K;蛋白酶 K ;肽链内切酶;;Endopeptidase KProk Protease KTritirachium album proteinase K
个人防护设备Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
WGK德国3
搜索质检报告(COA)
搜索MSDS
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