概述
Tyrosine decarboxylase catalyzes the removal of the carboxyl group from tyrosine to produce tyramine and carbon dioxide. Pyridoxal 5'-phosphate is a necessary cofactor. By using the apoenzyme prepared from cells grown on a vitamin B6 deficient medium pyridoxal phosphate may be determined. The HOLOenzyme may be used to determine tyrosine, phenylalanine and dihydroxyphenylalanine either manometrically or colorimetrically.
技术规格
| Source | Streptococcus faecalis (NCTC6783) |
| Description | APOenzyme. Dried cells grown in B6 deficient media. |
| Storage | Store at -20°C. |
| REQUIRES SPECIAL SHIPPING | DRY ICE |
| Activity | Activates to at least 0.2 units per mg |
| Unit Definitions | One Unit yields 1μmole of CO2per minute from L-tyrosine at 37°C, pH 5.5. The APOenzyme activity is measured in the presence of excess pyridoxal phosphate. |
党沛 
