| 存贮条件 | 储存温度 -20°C |
| 应用 | DNase II has been used to treat transformed cells during the purification of β-lactamase. It has also been used for the preparation of adenoma tissue in a study that investigated the effect of somatoprim on growth hormone secretion in human adenoma cell cultures (hSA).Deoxyribonuclease II from bovine spleen has been used in a study that conducted a partial purification of deoxyribonucleases from eggs and liver of Xenopus laevis. Deoxyribonuclease II from bovine spleen has also been used in a study to investigate nucleic acid and protein synthesis of splenic lymphocytes. |
| 产品介绍 | Deoxyribonuclease II from porcine spleen has a molecular weight of 38 kDa. The enzyme is a glycoprotein endonuclease with dimeric structure. Optimum pH range is 4.5-5.0 at ionic strength 0.15 M. Deoxyribonuclease II (Acid DNase) hydrolyzes deoxyribonucleotide linkages in native and denatured DNA yielding products with 3'-phosphates. It also acts on p-nitrophenylphosphodiesters at pH 5.6-5.9. Bernardi (BBRC, 17, 573, 1971) describes a three stage degradation of native DNA by DNase II. |
| 备注 | Chromatographically prepared. A solution in 50% glycerol. |
| 生化机理 | The enzyme is a lysosomal acid hydrolase that hydrolyzes DNA to 3′-phosphoryl oligonucleotides in the absence of metal ions, under acidic conditions. It is a non-covalently linked α·β heterodimer of approximately 45,000 Da molecular mass. |
| 别名 | ;DNase II;Deoxyribonucleate 3′-oligonucleotido-hydrolase |
脱氧核糖核酸酶Ⅱ 来源于猪脾(纯化,溶液)
Deoxyribonuclease II from porcine spleen(Purified,Solution) （订货以英文为准）
编号：D128605
CAS号：9025-64-3
分子式：232-801-8
| 产品名称 | Deoxyribonuclease II from porcine spleen(Purified,Solution) |
| 中文名称 | 脱氧核糖核酸酶Ⅱ 来源于猪脾(纯化,溶液) |
| CAS号 | 9025-64-3 |
| 分子式（M.F.） | 232-801-8 |
| 储存条件 | -20°C储存 |
| 个人防护设备 | Eyeshields,Gloves,type N95 (US),type P1 (EN143) respirator filter |
| RTECS | TX1760000 |
| WGK德国 | 3 |
| 安全声明（欧洲） | S24/25; |
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