| 存贮条件 | 储存温度 -20°C |
| 应用 | Nuclease S1 from Aspergillus oryzae has been used in a study to assess a biochemical method for mapping mutational alterations in DNA.It has also been used in a study to investigate the DNA damage and repair in a γ-irradiated rat brain tumor. |
| 产品介绍 | Nuclease S1 isolated from certain Neurospora and Aspergillus species specifically hydrolyzes both terminal and internal phosphodiester bonds of single-stranded DNA and RNA. Nuclease S1 has a molecular weight of approximately 34 kDa and exists as a monomer. The optimum pH range is 4.0-4.6, and it is activated by Zn2+ and/or Ca2+. Inhibitors are EDTA, citrate and high concentrations of SDS. |
| 备注 | Chromatographically purified. Specific for single-stranded DNA (ssDNA) degradation. Activity on native (ds) DNA undetectable under the assay conditions. A frozen solution in 30mM sodium acetate, pH 4.6, 50mM NaCl, 1mM ZnCl2, and 50% glycerol. |
| 生化机理 | Nuclease S1 isolated from Aspergillus oryzae exhibits endo- and exolytic hydrolytic activity for the phosphodiester bonds of single-stranded DNA and RNA yielding 5’-phosphomononucleotide and 5’-phosphooligonucleotide end-products. It is used to digest non-annealed polynucleotide tails and hairpin loops in RNA and DNA duplexes and can be used to convert superhelical DNA to the linear form. |
| 别名 | ;Endonuclease S1 |
核酸酶S1 来源于米曲霉
Nuclease S1 from Aspergillus oryzae （订货以英文为准）
编号：N128634
CAS号：37288-25-8
分子式：C103H206O8
分子量：1572.73
| 产品名称 | Nuclease S1 from Aspergillus oryzae |
| 中文名称 | 核酸酶S1 来源于米曲霉 |
| CAS号 | 37288-25-8 |
| 分子式（M.F.） | C103H206O8 |
| 分子量（M.W.） | 1572.73 |
| 储存条件 | -20°C储存 |
| Reaxys-RN | 92214 |
| 个人防护设备 | Eyeshields,Gloves,type N95 (US),type P1 (EN143) respirator filter |
| WGK德国 | 3 |
| 安全声明（欧洲） | S24/25; |
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