概述
Fusion protein corresponding to a region derived from 18-740 amino acids of human ACE2
技术规格
| Full name: | angiotensin I converting enzyme 2 |
| Synonyms: | ACEH |
| Swissprot: | Q9BYF1 |
| Purity: | >85%, as determined by Coomassie blue stained SDS-PAGE |
| Expression system: | Mammalian cell |
| Tags: | hFc tag C-terminals |
| Species: | Human |
| Molecular mass: | 111.1 kDa |
| Research areas: | Cell Biology, Cardiovascular |
| Activity: | 1.Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S (Catalog number: D140008) at 2 μg/ml can bind human ACE2, the EC50 of human ACE2 protein is 56.64 - 103.6 ng/ml 2.Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD (Catalog number: D140010) at 5 μg/ml can bind human ACE2, the EC50 of human ACE2 protein is 31.80 - 44.69 ng/ml. 3.Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD (Catalog number: D140012) at 5 μg/ml can bind human ACE2, the EC50 is 2.785-9.139 ng/ml. 4.Measured by its binding ability in a functional ELISA. Immobilized human ACE2 at 2 μg/ml can bind SARS-CoV-2-S1-RBD (Catalog number: D140011), the EC50 of human ACE2 protein is 8.363-12.82 ng/ml. 5.SARS-CoV-2 Spike protein RBD his/sumostar tag (Catalog number: D140010) captured on COOH chip can bind Human ACE2 protein Fc tag (Catalog number: D140021) with an affinity constant of 100 nM as detected by LSPR Assay. 6.SARS-CoV-2 Spike protein RBD his/myc tag (Catalog number: D140012) captured on COOH chip can bind Human ACE2 protein Fc tag (Catalog number: D140021) with an affinity constant of 13.8 nM as detected by LSPR Assay. |
| Endotoxin: | Less than 1.0 EU/ug as determined by LAL method. |
| Reconstitution: | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
| Background: | The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronavirus HCoV-NL63 and the human severe acute respiratory syndrome coronaviruses, SARS-CoV and SARS-CoV-2 (COVID-19 virus). |
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