概述
SPN? Protein Assay is a fast and efficient spin column method for protein estimation. It requires only 0.5‐10μg proteins per assay and no protein standards are needed. The assay is suitable for a wide range of protein samples including detergent solubilized membrane proteins and is compatible with common laboratory agents such as reducing sugars, thiols, chelating agents and detergents, including ≤2% SDS.
特点
- The concentration of protein is determined by comparing the optical density (OD595) data with the reference data on the supplied SPN? Tables. The protein concentration can be determined in <10 minutes. The SPN? Tables have been prepared using Bovine Serum Albumin (BSA) protein standards. For researcher’s convenience, we have included protein standard ODs for both spectrophotometer and microplate reader.
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