存贮条件 | 储存温度-20°C |
应用 | Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. It is also used in RNA sequence analysis and protection assays. |
产品介绍 | Pancreatic ribonuclease (RNase I) catalyzes cleavage of the phosphodiester bond between the 5'-ribose of a nucleotide and the phosphate group attached to the 3'-ribose of an adjacent pyrimidine nucleotide forming a 2',3'-cyclic phosphate which may then be hydrolyzed to the corresponding 3'-nucleoside phosphate. Ribonuclease A has a molecular weight of 13,700 daltons. It operates in an optimum pH range of 7.0-7.5.Ribonuclease B has a molecular weight of 14,700 ± 300 daltons. It is a glycoprotein which possesses an amino acid composition indistinguishable from that of RNase A. It contains 6 residues of mannose and 2 residues of N-acetylglucosamine per molecule. It is a glycosylated derivative of RNase A.Ribonuclease is inhibited by heavy metal ions and it is competitively inhibited by DNA. Since Molecular Biology Grade RNase A (Product R128619) is essentially free of DNase and protease activities, this product is useful in removing RNA from DNA in nucleic acid work and where other enzymes are used or where intact proteins must be recovered. |
备注 | Chromatographically purified. Aggregate-free. (Monophoretic on native gel electrophoresis.) A liquid in 100mM phosphate buffer containing 0.1% v/v phenol as a preservative. Note: The presence of 100mM phosphate makes this preparation unsuitable for heat treatment. |
别名 | RNase A;核糖核酸 3′-嘧啶寡核苷酸水解酶;核糖核酸酶 I;胰核糖核酸酶RNase A;Ribonuclease I;Pancreatic Ribonuclease;Ribonucleate 3′-pyrimidinooligonucleotidohydrolase |
核糖核酸酶A 来源于牛胰腺(纯化溶液)
Ribonuclease A from bovine pancreas(Purified Solution) (订货以英文为准)
编号:R128621
CAS号:9001-99-4
分子式:C9H14N4O3
分子量:226.23
产品名称 | Ribonuclease A from bovine pancreas(Purified Solution) |
中文名称 | 核糖核酸酶A 来源于牛胰腺(纯化溶液) |
CAS号 | 9001-99-4 |
分子式(M.F.) | C9H14N4O3 |
分子量(M.W.) | 226.23 |
储存条件 | -20°C储存 |
个人防护设备 | Eyeshields, Gloves |
WGK德国 | 3 |
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