| 存贮条件 | 储存温度 2-8°C |
| 应用 | Endoproteinase Glu-C from Staphylococcus aureus strain V8 is a serine protease used for selective cleavage of proteins for amino acid sequence determination or peptide mapping . |
| 产品介绍 | Protease S. aureus V8 (Endoproteinase-Glu-C) specifically cleaves peptide bonds on the COOH-terminal side of either aspartic or glutamic acids. In the presence of ammonium, the enzyme specificity is limited to glutamic sites. It has a molecular weight of 27,000 daltons and optimum pH's of 4.0 and 7.8 with hemoglobin as the substrate. Protease S. aureus V8 is inhibited by diisopropylfluorophosphate and monovalent anions such as F-, Cl-, CH3COO- and NO3. Enzyme activity is determined by the casein digestion assay described by Drapeau (Methods Enzymol., 45, 469, 1976). |
| 备注 | Chromatographically purified according to Drapeau, G., Boily, Y., and Houmard, J.: JBC, 247, 6720 (1972). A lyophilized powder. |
| 生化机理 | Staphylococcus strain V8 protease specifically cleaves peptide bonds on the carboxyl side of aspartic and glutamic acid residues when used in phosphate buffer. When used in ammonium bicarbonate buffer or ammonium acetate buffer cleavage is restricted to the carboxyl side of glutamic acid residues only. The enzyme exhibits maximal activity from pH 4.0 to 7.8. If hemoglobin is used as the substrate, maximal activity is at pH 4.0. The maximal activity is at pH of 7.8 when casein is the substrate. |
| 别名 | V8蛋白酶;V8 Protease;Protease,Staph aureus from Staphylococcus aureus V8 |
蛋白内切酶 Glu-C 来源于金黄色葡萄球菌 V8
Endoproteinase Glu-C from Staphylococcus aureus V8 （订货以英文为准）
编号：P128655
CAS号：66676-43-5
分子式：C65H98N16O19
分子量：1407.57
| 产品名称 | Endoproteinase Glu-C from Staphylococcus aureus V8 |
| 中文名称 | 蛋白内切酶 Glu-C 来源于金黄色葡萄球菌 V8 |
| CAS号 | 66676-43-5 |
| MDL编码 | MFCD01324998 |
| 分子式（M.F.） | C65H98N16O19 |
| 分子量（M.W.） | 1407.57 |
| 储存条件 | 2-8°C储存 |
| Merck Index | 7978 |
| 个人防护设备 | Eyeshields,Gloves,type N95 (US),type P1 (EN143) respirator filter |
| RTECS | UV1225000 |
| WGK德国 | 2 |
搜索质检报告(COA)
搜索MSDS
相关产品
¥ 103.90
T117926-25ml
¥ 1441.90
H128453-1g
¥ 2266.90
H128454-10UN
¥ 557.90
A118327-100mg
¥ 680.90
M139683-5KU
党沛 
