概述
Recombinant Mouse Beta-Nerve Growth Factor is produced by our E.coli expression system and the target gene encoding Ser122-Gly241 is expressed.
使用说明
This material is offered by Sangon Biotech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
技术规格
| Tag | None |
| 种属 | Mouse |
| 表达系统 | E.coli |
| Accession# | P01139 |
| Source | E.coli |
| Formulation_Description | Lyophilized from a 0.2 μm filtered solution of 20mM Tris, 150mM NaCl, pH 8.0. |
| Storage | Lyophilized protein should be stored at < -20°C, thoμgh stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
| Reconstitution | Dissolve the lyophilized protein in distilled water. |
| Purity | Greater than 95% as determined by reducing SDS-PAGE. |
| Endotoxin | Less than 0.1 ng/μg (1 EU/μg) as determined by LAL test. |
| Background | NGF is the first member discovered in the Neurotrophin family, which includes brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), and neurotrophin-4 (NT-4). These proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures. Mouse beta -NGF is a homodimer of two 120 amino acid polypeptides. It shares approximately 90% homology at the amino acid level with human beta -NGF and 95.8% with rat beta -NGF. NGF signaling has been shown to play an important role in neuroprotection and repair. β-NGF acts as a growth and differentiation factor for B lymphocytes, and enhances B-cell survival. It is a potent neurotrophic factor that signals throμgh its receptor β-NGFR, and plays a crucial role in the development and preservation of the sensory and sympathetic nervous systems. |
| Biological_Activity | Measured in a cell proliferation assay using TF 1 human erythroleukemic cells. The ED50 for this effect is 0.3-1.5 ng/ml. |
党沛 
