概述
Fusion protein corresponding to a region derived from 16-685 amino acids of human SARS-CoV-2 S protein
技术规格
| Full name: | Severe acute respiratory syndrome coronavirus 2 spike glycoprotein S1 |
| Synonyms: | COVID-19; 2019-nCoV; SARS-CoV-2; spike glycoprotein; 新型冠状病毒 |
| Swissprot: | P0DTC2 |
| Gene Accession: | MN908947 |
| Purity: | >90%, as determined by Coomassie blue stained SDS-PAGE |
| Expression system: | Yeast |
| Tags: | 6×His-sumostar tag N-terminals |
| Species: | SARS-CoV-2/2019-nCoV |
| Molecular mass: | 88.1 kDa |
| Research areas: | Microbiology |
| Reconstitution: | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
| Background: | Attaches the virion to the cell membrane by interacting with host receptor, initiating the infection (By similarity). Binding to human ACE2 receptor and internalization of the virus into the endosomes of the host cell induces conformational changes in the Spike glycoprotein (PubMed:32142651, PubMed:32075877, PubMed:32155444). Uses also human TMPRSS2 for priming in human lung cells which is an essential step for viral entry (PubMed:32142651). Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate membranes fusion within endosomes. |
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